A chemiluminescence method for determination of lipid hydroperoxides.
نویسندگان
چکیده
منابع مشابه
Method for the simultaneous determination of free/protein malondialdehyde and lipid/protein hydroperoxides.
A simple and sensitive method is presented for the simultaneous quantification (spectrophotometric and spectrofluorimetric) of the main lipid and protein peroxidation products after their initial fractionation: free malondialdehyde (FrMDA), protein-bound malondialdehyde (PrMDA), total hydroperoxides (LOOH), and protein hydroperoxides (PrOOH). FrMDA and PrMDA (released from proteins by alkaline ...
متن کاملA Sensitive Chemiluminescence Method for Determination of Hydroquinone and Catechol
A novel flow-injection chemiluminescence(CL)method has been developed for the determination of hydroquinone (HQ) and catechol (CT), based on their inhibition of the chemiluminescence reaction of luminol–KMnO4–β-cyclodextrin (β-CD). It was found that β-cyclodextrin could effectively enhance the chemiluminescence produced from the reaction of luminol with KMnO4 in basic media. The proposed method...
متن کاملTerbium Sensitized Chemiluminescence Method for the Determination of Rabeprazole -Application to Pharmaceutical Analysis and Dissolution Studies
A simple and sensitive chemiluminescence-based method was established for the determination of rabeprazole.The proposed method was based on the enhancing effect of rabeprazole on Ce(IV)-Na2SO3 -Tb(III) chemiluminescence reaction. A possible mechanism was discussed for chemiluminescence system by studying UV-Vis, fluorescence and chemiluminescence spectra. The effects of va...
متن کاملLipid hydroperoxides in plants.
Hydroperoxides are the primary oxygenated products of polyunsaturated fatty acids and were determined spectrophotometrically based on their reaction with an excess of Fe2+ at low pH in the presence of the dye Xylenol Orange. Triphenylphosphine-mediated hydroxide formation was used to authenticate the signal generated by the hydroperoxides. The method readily detected lipid peroxidation in a ran...
متن کاملPreparation of pure lipid hydroperoxides.
Increasing evidence of lipid peroxidation in food deterioration and pathophysiology of diseases have revealed the need for a pure lipid hydroperoxide (LOOH) reference as an authentic standard for quantification and as a compound for biological studies in this field. Generally, LOOH is prepared from photo- or enzymatically oxidized lipids; however, separating LOOH from other oxidation products a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Clinical Biochemistry and Nutrition
سال: 1990
ISSN: 1880-5086,0912-0009
DOI: 10.3164/jcbn.8.165